›› 2012, Vol. 24 ›› Issue (3): 179-182.doi: 10.3969/j.issn.1004-616x.2012.03.004

• 论著 • Previous Articles     Next Articles

Effect of 5-Aza-CdR on methylation and mRNA expression of RASSF1A genein nasopharyngeal carcinoma cells

YE Jing，LI Ming-hua，ZHANG Fang-ting，WAN Hui-juan，LONG Xia   

  1. Central Laboratory, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong, China
  • Received:2011-11-30 Revised:2012-02-05 Online:2012-05-30 Published:2012-05-30
  • Contact: YE Jing

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Abstract: OBJECTIVE: The relationship between methylation of RASSF1A gene and mRNA expression in nasopharyngeal carcinoma cell line CNE2 was investigated. METHODS：CNE2 cells were treated with various concentrations of 5-Aza-CdR. Methylation of RASSF1A gene was evaluated by methylation-specific polymerase chain reaction(MSP). Expression of RASSF1A mRNA was detected by SYBR Green qRealTime-PCR. RESULTS：RASSF1A gene was completely methylated in the negative control group cell. De-methylation occurred in the 5 μmol/L of 5-Aza-CdR. Methylation was completely reversed by 20 μmol/L of 5-Aza-CdR. The expression of RASSF1A mRNA in the negative control group was low. The expression level increased gradually by 5，10 and 20 μmol/L 5-Aza-CdR treatment. The levels in 10 and 20 μmol/L treated group were significantly improved in comparison with the negative control group (P<0.01). RASSF1A mRNA in 20 μmol/L treated group was obviously higher than that in 5 μmol/L treated group (P<0.01). CONCLUSION：Methylation of RASSF1A gene in the nasopharyngeal carcinoma cell line CNE2 could be reversed by 5-Aza-CdR， promoting the expression of RASSF1A gene mRNA.

Key words: nasopharyngeal carcinoma, CNE2, RASSF1A, methylation, expression

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